8WEG
Crystal structure of Brassica napus MIK2 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-01-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 156.820, 156.820, 125.633 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.940 - 2.850 |
| R-factor | 0.1988 |
| Rwork | 0.198 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.849 | 2.849 |
| Number of reflections | 40799 | 2249 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 97.1 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.980 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 289 | 0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 34% w/v PEG 400 |
