8WE2

14-3-3 zeta complexed with S609 phosphorylated peptide derived from GPIb alpha cytoplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	71.733, 129.192, 69.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.420 - 2.110
R-factor	0.1909
Rwork	0.189
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.827
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.420	2.220
High resolution limit [Å]	2.110	2.110
Rmerge	0.086
Number of reflections	37743	5433
<I/σ(I)>	12.7
Completeness [%]	99.6
Redundancy	5.9
CC(1/2)	0.997	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Sodium Acetate, 20% PEG 3350