8WD5

Crystal structure of farnesyl diphosphate synthase FPPS1 from silkworm, Bombyx mori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	63.530, 64.967, 108.378
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	42.365 - 2.800
Rwork	0.240
R-free	0.29230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.625
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.400	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.133	0.476
Rmeas	0.150	0.542
Rpim	0.069	0.253
Number of reflections	21918	3180
<I/σ(I)>	9.6
Completeness [%]	99.6	100
Redundancy	4.8
CC(1/2)	0.993	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2% Tacsimate pH 5.0, 0.1 M sodium citrate, pH 5.6, 16% polyethylene glycol 3350