8WAB
Crystal structure of a chondroitin sulfate-binding carbohydrate binding module of a chondroitinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.401, 45.717, 56.421 |
| Unit cell angles | 102.28, 96.00, 113.21 |
Refinement procedure
| Resolution | 27.550 - 2.200 |
| R-factor | 0.1739 |
| Rwork | 0.168 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.360 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.216 |
| Rmeas | 0.120 | 0.306 |
| Rpim | 0.085 | 0.216 |
| Total number of observations | 31219 | 2779 |
| Number of reflections | 17339 | 1533 |
| <I/σ(I)> | 5.3 | 2.7 |
| Completeness [%] | 96.2 | |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.960 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 27% PEG 4000, 0.1M Sodium cacodylate |
