8W9K
Structure of apo RvY_06210
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.273, 80.809, 105.547 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 40.400 - 1.700 |
| R-factor | 0.1863 |
| Rwork | 0.184 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.100 | 0.684 |
| Rmeas | 0.122 | 0.843 |
| Rpim | 0.068 | 0.547 |
| Number of reflections | 104042 | 5561 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 92.0 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.973 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4M Sodium thiocyanate, 0.1M Sodium acetate pH4.0, 16% w/v PEG 4000 |
