8W91

Azumapecten Farreri homopolymeric ferritin mutant - H2KE exposed to H2O2 for 3 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.6314
Spacegroup name	I 4 3 2
Unit cell lengths	153.716, 153.716, 153.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.610 - 2.120
R-factor	0.1587
Rwork	0.158
R-free	0.18350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.198
High resolution limit [Å]	2.120	2.122
Number of reflections	16848	1176
<I/σ(I)>	21.4
Completeness [%]	94.3
Redundancy	23.8
CC(1/2)	0.999	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, pH 7, 500 mM NaCl