8W8V

High-resolution X-ray structure of cellulase Cel6A from Phanerochaete chrysosporium at cryogenic temperature, Enzyme-Product complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	54.044, 67.581, 88.354
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 0.850
Rwork	0.105
R-free	0.11860
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	0.900
High resolution limit [Å]	0.850	0.850
Rmerge	0.068	0.733
Number of reflections	547722	82938
<I/σ(I)>	15.17
Completeness [%]	98.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	polyethylene glycol 3350, sodium chloride, cellotriose, acetate buffer,