8W8T

Crystal structure of human CLEC12A CRD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 61 2 2
Unit cell lengths	50.037, 50.037, 364.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.160 - 2.300
R-factor	0.2371
Rwork	0.235
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.420
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	2.380
High resolution limit [Å]	2.300	2.300
Rmeas	0.266	1.545
Rpim	0.062	0.343
Number of reflections	13302	1252
<I/σ(I)>	11.3	2.3
Completeness [%]	100.0	100
Redundancy	18.1
CC(1/2)	0.997	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Sodium cacodylate (pH 6.5), 0.2M Ammonium sulfate, 30% polyethylene glycol 8,000