8W8P

Thermus thermophilus initiation transcription complex containing CMPcPP in the post-translocated state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91810
Spacegroup name	C 1 2 1
Unit cell lengths	184.681, 103.891, 294.614
Unit cell angles	90.00, 98.97, 90.00

Refinement procedure

Resolution	39.755 - 3.165
R-factor	0.2125
Rwork	0.211
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.145
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.260
High resolution limit [Å]	3.165	3.200
Rmerge	0.244	0.938
Number of reflections	559327	559327
<I/σ(I)>	6.74	1.729
Completeness [%]	96.2
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.05M Magnesium chloride, 0.2M Potassium chloride, 0.1M Tris-HCl pH8.0, 10% PEG 4000