8W8O
Thermus thermophilus initiation complex in the half-translocated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.397, 103.796, 295.035 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 39.883 - 2.512 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.177 | 0.843 |
| Number of reflections | 1336849 | 1336849 |
| <I/σ(I)> | 11.699 | 2 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.05M Magnesium chloride, 0.2M Potassium chloride, 0.1M Tris-HCl pH8.0, 10% PEG 4000 |
