8W7F

Structure of Drosophila melanogaster L-2-hydroxyglutarate dehydrogenase bound with FAD and a sulfate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	174.518, 103.067, 122.876
Unit cell angles	90.00, 114.63, 90.00

Refinement procedure

Resolution	33.260 - 2.299
R-factor	0.2049
Rwork	0.204
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.549
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.299	6.240	2.300
Rmerge	0.147	0.064	0.959
Rmeas	0.159	0.069	1.076
Rpim	0.061	0.026	0.477
Number of reflections	86886	4465	4282
<I/σ(I)>	2.6
Completeness [%]	99.0	98.8	98.4
Redundancy	6.6	6.8	4.6
CC(1/2)	0.992	0.997	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	290	0.2 M lithium sulfate, 0.1 M Bis-Tris (pH 5.9), 23% (w/v) PEG 3350