8W75

Structure of Drosophila melanogaster L-2-hydroxyglutarate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	100.115, 103.178, 102.538
Unit cell angles	90.00, 108.61, 90.00

Refinement procedure

Resolution	31.910 - 2.850
R-factor	0.2458
Rwork	0.243
R-free	0.29440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.596
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.847	7.730	2.850
Rmerge	0.128	0.036	1.234
Rmeas	0.139	0.039	1.347
Rpim	0.053	0.015	0.530
Number of reflections	45678	2370	2075
<I/σ(I)>	4.5
Completeness [%]	98.4	98.6	89.9
Redundancy	6.7	6.6	5.7
CC(1/2)	0.992	0.999	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.2 M magnesium formate, 18% (w/v) PEG 3350