8W70

Structure of Gemtuzumab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	96.280, 125.740, 39.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.850 - 2.500
R-factor	0.1931
Rwork	0.192
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.294
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.850	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	17363	1620
<I/σ(I)>	14.4
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20% w/v PEG 8000 0.1 M HEPES pH 7.5