8W6U

Ferritin from Ureaplasma diversum soaking in Fe2+ solution for 5 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.55
Spacegroup name	F 4 3 2
Unit cell lengths	180.521, 180.521, 180.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.130 - 2.099
R-factor	0.1889
Rwork	0.184
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.845
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.099	5.700	2.100
Rmerge	0.200	0.093	1.102
Rmeas	0.238	0.094	1.112
Rpim	0.029	0.012	0.143
Number of reflections	27791	897	717
<I/σ(I)>	3.1
Completeness [%]	100.0	100	100
Redundancy	69.9	61.2	59.7
CC(1/2)	0.993	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	Bicine, MgCl2