8W6R

murine SMPDL3A bound to sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-10
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	86.374, 86.374, 79.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.540 - 1.950
R-factor	0.2229
Rwork	0.221
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.244
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.540	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.157	0.674
Number of reflections	42004	2041
<I/σ(I)>	27.6	1.6
Completeness [%]	97.7	96.6
Redundancy	9.1	5.1
CC(1/2)	0.986	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.1 M sodium citrate [pH 5.5], 0.2M ammonium sulfate and 30% PEG 4000