8W6Q
ferritin from Ureaplasma diversum soaking in Fe2+ solution for 0 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.55 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 179.814, 179.814, 179.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.954 - 2.101 |
R-factor | 0.1832 |
Rwork | 0.179 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.796 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 10.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.200 | 0.087 | 1.558 |
Rmeas | 0.212 | 0.088 | 0.990 |
Rpim | 0.026 | 0.011 | 0.224 |
Number of reflections | 27415 | 882 | 715 |
<I/σ(I)> | 3.3 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 20 | 15 | 15 |
CC(1/2) | 0.997 | 0.998 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Bicine, MgCl2 |