8W6M

Native strucutre of ferritin from Ureaplasma diversum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	F 4 3 2
Unit cell lengths	179.938, 179.938, 179.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.629 - 2.501
R-factor	0.1921
Rwork	0.186
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.114	0.043	0.512
Rmeas	0.116	0.044	0.520
Rpim	0.020	0.008	0.091
Total number of observations	304765
Number of reflections	9128	546	450
<I/σ(I)>	7.2
Completeness [%]	100.0	99.8	100
Redundancy	33.4	28	32.1
CC(1/2)	0.993	1.000	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Bicine, MgCl2