8W6L
Crystal structure of the SLA-2*1001 allele and ASFV antigenic peptide at 2.2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.531, 48.883, 51.173 |
| Unit cell angles | 94.40, 101.86, 100.92 |
Refinement procedure
| Resolution | 49.720 - 2.030 |
| R-factor | 0.23964 |
| Rwork | 0.238 |
| R-free | 0.27652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.622 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.720 | 2.580 |
| High resolution limit [Å] | 2.029 | 2.300 |
| Rmerge | 0.022 | |
| Rpim | 0.142 | |
| Number of reflections | 24852 | 1790 |
| <I/σ(I)> | 2.44 | 3.78 |
| Completeness [%] | 98.0 | |
| Redundancy | 1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium thiocyanate,20% w/v Polyethylene glycol 3,350,pH 6.9 |
