8W6B

crystal structure of TAX1BP1 SKICH domain in complex with RB1CC1 coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97890
Spacegroup name	P 1 21 1
Unit cell lengths	40.239, 86.815, 147.860
Unit cell angles	90.00, 95.74, 90.00

Refinement procedure

Resolution	42.700 - 2.390
R-factor	0.1952
Rwork	0.193
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.360	2.520
High resolution limit [Å]	2.390	2.390
Number of reflections	39479	5914
<I/σ(I)>	13.8
Completeness [%]	97.7
Redundancy	6.7
CC(1/2)	0.998	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	5% (w/v) PEG 4000, 50 mM Magnesium chloride, 100 mM MES at pH 6.5, 10% 2-propanol