8W5S

Crystal structure of Annexin A7 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 65
Unit cell lengths	89.813, 89.813, 196.916
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.900 - 2.116
Rwork	0.257
R-free	0.31510
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.116	2.116
Number of reflections	51079	20158
<I/σ(I)>	3.8
Completeness [%]	99.8
Redundancy	9.04
CC(1/2)	0.958	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M Calcium chloride dihydrate, 20% PEG 3350