8W3R

Crystal structure of prefusion-stabilized RSV F protein UFCR2-iSS-P2-NQ in complex with A4 single-chain fragment variable

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	H 3 2
Unit cell lengths	100.240, 100.240, 588.218
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.120 - 4.020
R-factor	0.2516
Rwork	0.249
R-free	0.30260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.120	4.167
High resolution limit [Å]	4.020	4.020
Number of reflections	9477	742
<I/σ(I)>	16.9
Completeness [%]	94.8
Redundancy	13.2
CC(1/2)	0.998	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH 5.87, 10 %(w/v) PEG 6,000.