8W3Q

Crystal structure of prefusion-stabilized hMPV F protein UFCM1-P2-iSS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	I 21 3
Unit cell lengths	185.745, 185.745, 185.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.640 - 5.990
R-factor	0.23
Rwork	0.224
R-free	0.35460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.380
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	6.215
High resolution limit [Å]	5.990	5.990
Number of reflections	2793	278
<I/σ(I)>	22.42
Completeness [%]	99.6
Redundancy	38.2
CC(1/2)	0.999	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4M potassium dihydrogen phosphate, 1.2M sodium dihydrogen phosphate, 0.1M phosphate-citrate pH4.37