8W26
X-ray crystal structure of the GAF-PHY domains of SyB-Cph1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-06 |
Detector | MAR CCD 300 mm |
Wavelength(s) | 0.9787 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.846, 102.846, 130.516 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.570 - 2.600 |
R-factor | 0.2047 |
Rwork | 0.201 |
R-free | 0.24230 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.610 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.074 | 0.023 | 1.516 |
Rmeas | 0.083 | 0.027 | 1.710 |
Rpim | 0.038 | 0.013 | 0.780 |
Total number of observations | 192826 | ||
Number of reflections | 40901 | 2051 | 2044 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 99.9 | 98.7 | 100 |
Redundancy | 4.7 | 4.5 | 4.7 |
CC(1/2) | 0.998 | 0.999 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tacsimate, pH 7.0, MOPS, pH 7.0, PEG 3350, DMSO |