8W26

X-ray crystal structure of the GAF-PHY domains of SyB-Cph1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-06
Detector	MAR CCD 300 mm
Wavelength(s)	0.9787
Spacegroup name	P 43 21 2
Unit cell lengths	102.846, 102.846, 130.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.570 - 2.600
R-factor	0.2047
Rwork	0.201
R-free	0.24230
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.610
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.074	0.023	1.516
Rmeas	0.083	0.027	1.710
Rpim	0.038	0.013	0.780
Total number of observations	192826
Number of reflections	40901	2051	2044
<I/σ(I)>	8.1
Completeness [%]	99.9	98.7	100
Redundancy	4.7	4.5	4.7
CC(1/2)	0.998	0.999	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Tacsimate, pH 7.0, MOPS, pH 7.0, PEG 3350, DMSO