8W21

Crystal Structure of Enolase from Chlamydia trachomatis (P43212 Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-03
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 43 21 2
Unit cell lengths	140.602, 140.602, 409.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 2.250
R-factor	0.1773
Rwork	0.175
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_5243)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	2.310
High resolution limit [Å]	2.250	2.250
Rmerge	0.191	1.524
Rmeas	0.199	1.605
Rpim	0.054	0.484
Total number of observations	2569312	135568
Number of reflections	192780	13148
<I/σ(I)>	11.3	1.5
Completeness [%]	99.3
Redundancy	13.3	10.3
CC(1/2)	0.997	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	Berkeley D7: 100 mM Potassium phosphate dibasic, 100 mM potassium chloride, 30% Peg 3350, ChtrB.00084.a.B1.PW39241 at 18.9 mg/mL. plate 13805 well D7 drop 2. Puck: PSL-0301, Cryo: 20% PEG 200 + 80% crystallant