8W0T

Human LCAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-01-17
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	101.143, 102.052, 173.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.130 - 2.500
R-factor	0.2
Rwork	0.200
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge		0.281
Number of reflections	52001	2227
<I/σ(I)>	15.5
Completeness [%]	83.0	36
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	291	TrisHCl, PEG5000MME, NaCl, MgCl2