8W02
Q108K:K40L:T51V:T53S:R58W mutant of hCRBPII bound to synthetic fluorophore TD-1V
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.889, 36.209, 64.081 |
| Unit cell angles | 90.32, 91.86, 113.62 |
Refinement procedure
| Resolution | 27.360 - 1.499 |
| Rwork | 0.207 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qyn |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.776 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.360 | 1.553 |
| High resolution limit [Å] | 1.499 | 1.499 |
| Rmeas | 0.052 | 0.555 |
| Rpim | 0.027 | 0.281 |
| Number of reflections | 39448 | 11993 |
| <I/σ(I)> | 20.8 | 3.24 |
| Completeness [%] | 96.4 | 94.4 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.882 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | ammonium acetate, PEG 4000, sodium acetate |
