8VZ9

Crystal structure of mouse MAIT M2A TCR-MR1-5-OP-RU complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 2 21 21
Unit cell lengths	64.531, 84.259, 177.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.220 - 3.400
R-factor	0.2445
Rwork	0.242
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	3.520
High resolution limit [Å]	3.400	3.400
Number of reflections	13899	1346
<I/σ(I)>	10.12
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.995	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bis-Tris Propane (pH 7.5 - 8.5), 16-22% PEG 3350 and 0.2M sodium acetate