8VZ8

Crystal structure of mouse MAIT M2B TCR-MR1-5-OP-RU complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95365
Spacegroup name	P 2 21 21
Unit cell lengths	64.486, 84.635, 166.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.640 - 3.450
R-factor	0.2585
Rwork	0.256
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.577
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.640	3.570
High resolution limit [Å]	3.450	3.450
Number of reflections	12522	1235
<I/σ(I)>	4.29
Completeness [%]	99.8
Redundancy	4.29
CC(1/2)	0.977	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bis-Tris Propane (pH 7.5 - 8.5), 16-22% PEG 3350 and 0.2M sodium acetate