8VY6

Murine light chain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 2
Unit cell lengths	123.679, 132.648, 65.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.220 - 2.800
R-factor	0.20095
Rwork	0.198
R-free	0.25922
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.304
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.082	0.627
Rmeas	0.096	0.754
Rpim	0.049	0.410
Number of reflections	13125	639
<I/σ(I)>	17.5	2.1
Completeness [%]	95.6	94.4
Redundancy	3.4
CC(1/2)		0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	The crystals were obtained when a 5 mg/mL protein sample was mixed in a 1:1 volume ratio with a well solution composed of 0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5, and 25% w/v polyethylene glycol 3,350.