8VY5

Crystal structure of human SAE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	CCD
Collection date	2024-03-21
Detector	MAR CCD 300 mm
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	103.197, 70.187, 87.591
Unit cell angles	90.00, 108.92, 90.00

Refinement procedure

Resolution	28.290 - 2.010
R-factor	0.2327
Rwork	0.231
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.231
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.990	2.120
High resolution limit [Å]	2.010	2.010
Rmerge	0.053	1.141
Rmeas	0.064
Rpim	0.035	0.918
Number of reflections	38233	5741
<I/σ(I)>	13.2	1.1
Completeness [%]	96.4
Redundancy	6.1
CC(1/2)	0.995	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	Tris 8.5, PEG3350, ammonium acetate NDSB-256