8VX3
Structure of an exported small alarmone synthetase from Streptomyces albidoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.939, 133.059, 68.050 |
| Unit cell angles | 90.00, 89.77, 90.00 |
Refinement procedure
| Resolution | 47.570 - 2.400 |
| R-factor | 0.2408 |
| Rwork | 0.238 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.406 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.127 | 1.290 |
| Rmeas | 0.143 | 1.473 |
| Rpim | 0.064 | 0.692 |
| Number of reflections | 53335 | 2646 |
| <I/σ(I)> | 12.86 | 1.11 |
| Completeness [%] | 98.6 | 97.1 |
| Redundancy | 4.4 | |
| CC(1/2) | 0.990 | 0.418 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 0.1M imidazole-HCl pH 8, 1M sodium citrate |
