8VWO
Crystal structure of hRpn13 Pru bound with U-shaped peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.262, 56.974, 77.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.850 |
| R-factor | 0.1961 |
| Rwork | 0.195 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | DIALS |
| Refinement software | REFMAC (REFMAC5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.238 |
| Rmeas | 0.080 | 0.258 |
| Rpim | 0.031 | 0.099 |
| Number of reflections | 12367 | 717 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 6.4 | 6.7 |
| CC(1/2) | 0.998 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 277.15 | 0.1 M sodium acetate pH 4.6 and 22% PEG3350 |
