8VWN

Crystal structure of Vibrio cholerae NFeoB in the GDP-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2023-02-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12706
Spacegroup name	C 1 2 1
Unit cell lengths	194.270, 155.309, 50.916
Unit cell angles	90.00, 95.88, 90.00

Refinement procedure

Resolution	50.650 - 4.250
R-factor	0.2235
Rwork	0.221
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.763
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.530	4.330
High resolution limit [Å]	4.250	4.250
Number of reflections	10461	447
<I/σ(I)>	6.9
Completeness [%]	98.8
Redundancy	3.1
CC(1/2)	0.983	0.356

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG 3350, 0.2 M magnesium chloride, 0.1 M bis-tris pH 5.5, 0.1 M lithium chloride, 3 mM GDP