8VVL
CCHFV GP38 bound to c13G8 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 100.429, 67.629, 140.983 |
| Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
| Resolution | 45.160 - 1.800 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.752 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 1.868 |
| High resolution limit [Å] | 1.800 | 1.804 |
| Rmerge | 0.033 | 0.269 |
| Rmeas | 0.047 | 0.380 |
| Rpim | 0.033 | 0.269 |
| Number of reflections | 81877 | 8008 |
| <I/σ(I)> | 9.5 | 2.5 |
| Completeness [%] | 94.5 | 92.89 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.998 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 2 M Ammonium sulfate, 0.1 M Bis-Tris pH 5.5, 0.01 M Cobalt chloride hexahydrate |
