8VVJ

Structure of S. odontolytica ZTP riboswitch bound to m-1-pyridinyl-AICA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 6 2 2
Unit cell lengths	118.247, 118.247, 202.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	101.030 - 2.150
R-factor	0.3126
Rwork	0.312
R-free	0.33390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.918
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.030	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.255	2.508
Number of reflections	85958	4513
<I/σ(I)>	12.41
Completeness [%]	99.9
Redundancy	39.1
CC(1/2)	0.997	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	125-300 mM lithium sulfate, 23-27% PEG 3350, 0.1 M Hepes pH 7.5