8VUD

Crystal structure of APOBEC3F-CD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033202
Spacegroup name	P 6
Unit cell lengths	124.388, 124.388, 64.011
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.213 - 2.599
R-factor	0.1844
Rwork	0.178
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.308
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.599	5.600	2.600
Rmerge	0.146	0.074	0.882
Rmeas		0.076	0.915
Rpim		0.018	0.239
Number of reflections	17567	1821	1751
<I/σ(I)>	8.2
Completeness [%]	99.9	100	100
Redundancy	17.1	18.1	14.3
CC(1/2)		0.999	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis-Tris, pH 5.5, 1.8 M ammonium sulfate