8VTL

Crystal structure of R. sphaeroides Photosynthetic Reaction Center variant Y(M210)2-methoxyphenylalanine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	250
Detector technology	PIXEL
Collection date	2023-08-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97893
Spacegroup name	P 31 2 1
Unit cell lengths	141.430, 141.430, 187.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.890 - 3.050
R-factor	0.1826
Rwork	0.181
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.890	3.160
High resolution limit [Å]	3.050	3.050
Rmerge	0.211	1.849
Rmeas	0.216	1.987
Rpim	0.065	0.561
Number of reflections	41821	4111
<I/σ(I)>	13.35	1.53
Completeness [%]	99.9	99.98
Redundancy	11.3	11.8
CC(1/2)	0.998	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1 M potassium phosphate, 3.5% 1,2,3-heptanetriol, and 0.1% LDAO precipitant solution; 1.4-1.5 M potassium phosphate reservoir solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K