8VTH
DUF2169 domain-containing protein from Vibrio xiamenensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97859 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.350, 56.630, 69.510 |
| Unit cell angles | 83.27, 88.72, 66.72 |
Refinement procedure
| Resolution | 30.400 - 1.850 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.920 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.071 | 1.325 |
| Number of reflections | 53211 | 1298 |
| <I/σ(I)> | 13.5 | 1.3 |
| Completeness [%] | 84.0 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.999 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 22% PEG3350 2% Benzamidine HCl 15mM Ammonium sulfate |
