8VT5
Crystal structure of human menRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-18 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 48.779, 48.779, 288.169 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.030 - 2.690 |
| R-factor | 0.2524 |
| Rwork | 0.250 |
| R-free | 0.29120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.030 | 2.787 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 6340 | 577 |
| <I/σ(I)> | 6.38 | |
| Completeness [%] | 95.8 | |
| Redundancy | 14.2 | |
| CC(1/2) | 0.992 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.09 M NPS (0.3M Sodium Nitrate, 0.3M Sodium phosphate dibasic, 0.3M Ammonium sulfate), 0.5 M of a mix consisting of 1 M Sodium HEPES and MOPS (pH 7.5), and 30% from a mix consisting of 40% PEG 500 and 20% PEG 20000 |
