8VS8

Crystal structure of ADI-19425 Fab in complex with anti-idiotypic 1D3 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00003
Spacegroup name	P 1
Unit cell lengths	95.069, 149.981, 164.430
Unit cell angles	83.98, 89.90, 74.61

Refinement procedure

Resolution	49.330 - 2.670
R-factor	0.2327
Rwork	0.231
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.450	2.720
High resolution limit [Å]	2.670	2.670
Rmerge	0.109	0.860
Rmeas	0.155	1.216
Rpim	0.109	0.860
Total number of observations	434021	21591
Number of reflections	228774	11390
<I/σ(I)>	4	0.7
Completeness [%]	92.5
Redundancy	1.9	1.9
CC(1/2)	0.961	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M MES pH 6.5 12% PEG 20K 4% formamide