8VS5

Structure of catalytic domain of telomere resolvase, ResT, from Borrelia garinii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	85.172, 85.172, 110.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.830 - 2.796
R-factor	0.1734
Rwork	0.170
R-free	0.20320
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.796	2.796
Number of reflections	18265	10253
<I/σ(I)>	20.3
Completeness [%]	96.9
Redundancy	23.6
CC(1/2)	0.643	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.5M ammonium sulfate, 0.1M Tris-HCl pH 8.5, 12% glycerol