8VRV

GII.4c H2-tri HBGA norovirus protruding domain

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	76.666, 90.470, 91.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.670 - 1.610
R-factor	0.1579
Rwork	0.157
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	1.640
High resolution limit [Å]	1.610	1.610
Rmerge	0.077	0.797
Rmeas	0.083	0.862
Rpim	0.032	0.326
Total number of observations	562066	26494
Number of reflections	82708	3989
<I/σ(I)>	11.7	1.7
Completeness [%]	99.9
Redundancy	6.8	6.6
CC(1/2)	0.993	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	SG1 1-24 0.2 M Magnesium formate dihydrate 20% w/v PEG 3350