8VRF
Sucrose-phosphate synthase-like protein from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953733 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.057, 92.709, 100.550 |
| Unit cell angles | 90.00, 120.44, 90.00 |
Refinement procedure
| Resolution | 48.080 - 1.740 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.637 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.052 | 0.612 |
| Rmeas | 0.062 | 0.722 |
| Rpim | 0.033 | 0.379 |
| Number of reflections | 104260 | 4988 |
| <I/σ(I)> | 13.8 | 2.1 |
| Completeness [%] | 99.0 | 95.8 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.999 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 26% PEG 3350, 200 mM Magnesium Chloride, 1mM UDPG, Protein crystal seeds |
