8VQV

Structure of S. odontolytica ZTP riboswitch bound to m-1-pyridinyl-AICA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.105030
Spacegroup name	P 43 21 2
Unit cell lengths	41.035, 41.035, 237.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.440 - 2.430
R-factor	0.2116
Rwork	0.207
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.440	2.517
High resolution limit [Å]	2.430	2.430
Rmerge	0.097
Number of reflections	14549	788
<I/σ(I)>	16.27
Completeness [%]	99.6
Redundancy	12
CC(1/2)	0.998	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	TBD