8VQP

Crystal Structure Analysis of EGR1-MIDN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	I 21 21 21
Unit cell lengths	49.460, 70.060, 84.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.390 - 2.520
R-factor	0.2451
Rwork	0.244
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.193
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.390	2.590
High resolution limit [Å]	2.520	2.520
Rmerge	0.078	2.392
Rmeas	0.084	2.589
Rpim	0.031	0.975
Total number of observations	37758	2482
Number of reflections	5217	367
<I/σ(I)>	13.7	0.7
Completeness [%]	99.9
Redundancy	7.2	6.8
CC(1/2)	0.999	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100mM HEPES, pH 7.0 1.4M Nh4SO4