8VPO
X-Ray Crystal Structure of TigE from Paramaledivibacter caminithermalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.172, 74.348, 91.765 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.880 - 1.660 |
R-factor | 0.1662 |
Rwork | 0.165 |
R-free | 0.19190 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.431 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | autoSHARP |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.880 | 1.700 |
High resolution limit [Å] | 1.660 | 1.660 |
Number of reflections | 56752 | 4002 |
<I/σ(I)> | 19.2 | 3.1 |
Completeness [%] | 99.7 | |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 17% w/v PEG 10,000 and equilibrating against 0.5 M LiCl |