8VOL

Apex domain deletion mutant of bacteriophage P2 central spike protein, membrane-piercing module

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.774900
Spacegroup name	P 1
Unit cell lengths	45.520, 49.910, 72.930
Unit cell angles	91.61, 88.25, 111.65

Refinement procedure

Resolution	26.730 - 0.900
R-factor	0.1164
Rwork	0.116
R-free	0.12700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.078
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.730	0.930
High resolution limit [Å]	0.900	0.900
Rmeas	0.083	0.544
Number of reflections	821847	55324
<I/σ(I)>	9.69	2.07
Completeness [%]	94.5	85.9
Redundancy	3.33	3.15
CC(1/2)	0.995	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	26-33% Pentaerythritol ethoxylate (15/4 EO/OH) aka PEE-797, 100 mM Bis-Tris or MES pH 6.0-6.5