8VOL
Apex domain deletion mutant of bacteriophage P2 central spike protein, membrane-piercing module
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.774900 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.520, 49.910, 72.930 |
| Unit cell angles | 91.61, 88.25, 111.65 |
Refinement procedure
| Resolution | 26.730 - 0.900 |
| R-factor | 0.1164 |
| Rwork | 0.116 |
| R-free | 0.12700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.078 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.730 | 0.930 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmeas | 0.083 | 0.544 |
| Number of reflections | 821847 | 55324 |
| <I/σ(I)> | 9.69 | 2.07 |
| Completeness [%] | 94.5 | 85.9 |
| Redundancy | 3.33 | 3.15 |
| CC(1/2) | 0.995 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 26-33% Pentaerythritol ethoxylate (15/4 EO/OH) aka PEE-797, 100 mM Bis-Tris or MES pH 6.0-6.5 |
