8VNP
Homing endonuclease I-PpoI-DNA complex at pH6.0 (K+ MES) with 0.2 M sodium malonate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-07-31 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.1272 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.695, 113.695, 87.994 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.280 - 1.790 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.18980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.956 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.280 | 34.280 | 1.890 |
High resolution limit [Å] | 1.790 | 5.330 | 1.790 |
Rmerge | 0.048 | 0.033 | 0.151 |
Rmeas | 0.051 | 0.035 | 0.159 |
Number of reflections | 120708 | 4544 | 19436 |
<I/σ(I)> | 33.42 | ||
Completeness [%] | 99.8 | ||
Redundancy | 20.5 | ||
CC(1/2) | 1.000 | 0.999 | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |