8VNA
Homing endonuclease I-PpoI-DNA complex:reaction at pH8.0 (Tris) with 500 uM Mg2+ for 160s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-04-05 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.650, 113.650, 88.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.200 - 1.540 |
R-factor | 0.1826 |
Rwork | 0.182 |
R-free | 0.19720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.172 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.200 | 37.200 | 1.640 |
High resolution limit [Å] | 1.540 | 4.610 | 1.540 |
Rmerge | 0.076 | 0.040 | 0.819 |
Rmeas | 0.084 | 0.044 | 0.904 |
Number of reflections | 188691 | 7088 | 29929 |
<I/σ(I)> | 11.94 | ||
Completeness [%] | 99.6 | ||
Redundancy | 11 | ||
CC(1/2) | 0.998 | 0.998 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |