8VMY
Homing endonuclease I-PpoI-DNA complex:reaction at pH6.0 (K+ MES) with 500 uM Mg2+ for 20s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-31 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9765 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.880, 113.880, 88.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.840 - 1.530 |
R-factor | 0.1804 |
Rwork | 0.180 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.155 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.840 | 47.840 | 1.620 |
High resolution limit [Å] | 1.530 | 4.560 | 1.530 |
Rmerge | 0.081 | 0.041 | 0.647 |
Rmeas | 0.091 | 0.046 | 0.727 |
Number of reflections | 193002 | 7254 | 30560 |
<I/σ(I)> | 10.27 | ||
Completeness [%] | 99.5 | ||
Redundancy | 9.7 | ||
CC(1/2) | 0.998 | 0.998 | 0.767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |